Coupling of Termination, 3′ Processing, and mRNA Export
نویسندگان
چکیده
منابع مشابه
Coupling of termination, 3' processing, and mRNA export.
In a screen to identify genes required for mRNA export in Saccharomyces cerevisiae, we isolated an allele of poly(A) polymerase (PAP1) and novel alleles encoding several other 3' processing factors. Many newly isolated and some previously described mutants (rna14-48, rna14-49, rna14-64, rna15-58, and pcf11-1 strains) are defective in polymerase II (Pol II) termination but, interestingly, retain...
متن کاملConnections between mRNA 3' end processing and transcription termination.
Discoveries within the last few years have revealed that the multiple steps in gene expression are remarkably integrated. There have recently been several advances in deciphering how mRNA 3' end processing is linked with transcription elongation and termination. It has been known for quite a long time that transcription termination is somehow intertwined with polyadenylation, but it is still un...
متن کاملCoupling transcription, splicing and mRNA export.
Previous studies have led to the view that mRNAs are transported from the nucleus to the cytoplasm by machinery that is conserved from yeast to humans. Moreover, this machinery is coupled both physically and functionally to the pre-mRNA splicing machinery. During the past year, important new insights into the mechanisms behind this coupling have been made. In addition, recent advances have reve...
متن کاملThe role of Rat1 in coupling mRNA 3'-end processing to transcription termination: implications for a unified allosteric-torpedo model.
The torpedo model of transcription termination by RNA polymerase II proposes that a 5'-3' RNA exonuclease enters at the poly(A) cleavage site, degrades the nascent RNA, and eventually displaces polymerase from the DNA. Cotranscriptional degradation of nascent RNA has not been directly demonstrated, however. Here we report that two exonucleases, Rat1 and Xrn1, both contribute to cotranscriptiona...
متن کاملMammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export.
Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between mRNA 3' end formation and export, it is not known how these two processes are mechanistically connected. Here, we show that a subunit of the mammalian pre-mRNA 3' end processing complex, CF I(m)68, stimulates mRNA export. CF I(m...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 2002
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.22.18.6441-6457.2002